ginkgo-cloud-lab

Files (19)

• SKILL.md
• cell-free-protein-expression-hibit.md · references/
• cell-free-protein-expression-optimization.md · references/
• cell-free-protein-expression-validation.md · references/
• cfps-expression-purification-quantification.md · references/
• cfps-strep-purification-thermal-shift.md · references/
• cfps-strep-tag-purification-a280.md · references/
• echo-ms-cfps-detection.md · references/
• echo-ms-method-onboarding.md · references/
• ecoli-expression-purification-quantification.md · references/
• ecoli-minibinder-expression-histag-a280.md · references/
• ecoli-protein-expression-hibit.md · references/
• ecoli-protein-expression-histag-a280.md · references/
• fluorescent-pixel-art-generation.md · references/
• ivt-rna-synthesis-qpcr.md · references/
• minibinder-strep-tag-a280.md · references/
• pichia-protein-expression-labchip.md · references/
• plate-reader-assay-onboarding.md · references/
• spr-target-onboarding.md · references/

SKILL.md (first lines)

# Ginkgo Cloud Lab

## Overview

Ginkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols use Reconfigurable Automation Carts (RACs), modular units with robotic arms and plate transport, across a catalog-advertised fleet of 70+ integrated instruments.

The platform also includes **EstiMate**, a compatibility and pricing assistant that accepts protocol descriptions, files, or links and returns preliminary estimates for custom workflows.

The catalog is organized into **Expression & Purification** (in vitro / cell-free / E. coli / Pichia), **Characterization & Assay**, **Method & Target Onboarding**, and **Specialty**. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details.

## Available Protocols

The following prices, availability labels, and turnaround text are a **2026-09-30
catalog snapshot**, not a configured quote. References link to the corresponding
service terms. Catalog and terms sometimes disagree on days versus business days,
input format, or readout scope; resolve those differences in the service order.

### Expression & Purification - In vitro

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [IVT mRNA/circRNA Synthesis](references/ivt-rna-synthesis-qpcr.md) | qPCR (mRNA or circRNA, 384-well) | $99/sample | up to 12 business days | Certified |

### Expression & Purification - Cell-free (E. coli CFPS)

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [Validate sequence expression](references/cell-free-protein-expression-validation.md) | Go/no-go titer + purity (up to 1800 bp) | $39/sample | up to 10 days | Certified |
| [Optimize expression conditions](references/cell-free-protein-expression-optimization.md) | DoE across 24 conditions | $199/sample | up to 11 days | Certified |
| [Express + quantify (HiBiT)](references/cell-free-protein-expression-hibit.md) | Luminescence, no purification | $39/sample | up to 11 days | Certified |
| [Express + purify (A280)](references/cfps-strep-tag-purification-a280.md) | Strep-tag, A280 yield | $149/sample | up to 11 days | Certified |
| [Express + purify minibinder](references/minibinder-strep-tag-a280.md) | Strep-tag, A280; confirm LabChip separately | $149/sample | up to 11 days | Certified |
| [Express + purify (A280 + LabChip)](references/cfps-expression-purification-quantification.md) | Strep-tag, A280 + purity/size | $159/sample | up to 12 days | Certified |

### Expression & Purification - E. coli

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [Express + quantify (HiBiT)](references/ecoli-protein-expression-hibit.md) | Luminescence (up to 384 constructs) | $79/sample | up to 3 weeks | Certified |
| [Express + purify (A280)](references/ecoli-protein-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified |
| [Express + purify minibinder](references/ecoli-minibinder-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified |
| [Express + purify (A280 + LabChip)](references/ecoli-expression-purification-quantification.md) | His-tag, A280 + purity/size | $209/sample | up to 3 weeks | Certified |

### Expression & Purification - Pichia

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [Express + quantify (LabChip)](references/pichia-protein-expression-labchip.md) | Secreted protein, size/purity (up to 96) | $89/sample | up to 4 weeks | Certified |

### Characterization & Assay

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [Express + thermal shift](references/cfps-strep-purification-thermal-shift.md) | SYPRO Orange Tm (Tonset, TM1-3) | $159/sample | up to 12 days | Certified |
| [Detect enzymatic products (Echo-MS)](references/echo-ms-cfps-detection.md) | Substrate/product by Echo-MS | $44/sample | up to 13 days | Beta |

### Method & Target Onboarding

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [Onboard Echo-MS method](references/echo-ms-method-onboarding.md) | Calibration curve, LOD/LOQ | $799/molecule | up to 3 weeks | Certified |
| [Onboard SPR target](references/spr-target-onboarding.md) | Validated SPR capture method | $1,399/target | up to 4 weeks | Beta |
| [Onboard plate-reader assay](references/plate-reader-assay-onboarding.md) | Qualification data; customer assesses performance | $399/assay | up to 4 weeks | Certified |

### Specialty

| Protocol | Readout | Price | Turnaround | Status |
|---|---|---|---|---|
| [Generate fluorescent pixel art](references/fluorescent-pixel-art-generation.md) | UV photo, 7-color E. coli palette | $25/plate | up to 7 days | Beta |

**Coming soon:** Protein Expression and Binding Affinity Characterization (express + purify, then screen binding affinity against a target).

## Choosing a Protocol

- **Quick expressibility screen?** Cell-free HiBiT ($39) or Validate sequence expression ($39).
- **Need purified protein + yield?** A280 tiers (cell-free or E. coli); add LabChip for purity/size.
- **Difficult / membrane / disulfide / cofactor targets?** Cell-free Optimize (24-condition DoE).
- **Secreted or eukaryotic targets?** Pichia expression.
- **Screening de novo binders/minibinders?** Expression tiers screen yield; SPR onboarding qualifies the target. Confirm availability of the separate downstream binding service before planning kinetics.
- **Enzyme activity / biocatalysis?** Echo-MS enzymatic detection (onboard the analyte method first).
- **Stability / developability ranking?** Thermal shift assay.
- **RNA (mRNA/circRNA)?** IVT synthesis + qPCR.
- **Transfer an existing plate-reader assay?** Plate-reader onboarding; check its single-factor, 96-well, fluorescence scope before preparing the intake.

## General Ordering Workflow

Treat the tables above as planning estimates. Recheck the selected protocol's
[current catalog page](https://cloud.ginkgo.bio/protocols) and configured quote
for the actual sample count, replicates, readout, and turnaround before ordering.
Save the protocol URL, downloaded input-template revision, submitted construct
manifest, replicate/plate map, quote identifier, and access date together. A
feasibility report or quote is not evidence that execution has started or that
results passed QC.

1. Select a protocol at https://cloud.ginkgo.bio/protocols
2. Configure parameters (number of proteins/samples/molecules/targets, replicates, plates)
3. Download the template linked on that protocol page and inspect its actual format and fields. Broad upload extensions do not define the intake schema. Keep construct IDs, sequence type, tag/linker, replicate count, and plate mapping explicit; distinguish technical replicates from independent expression reactions.
4. Add any special requirements in the Additional Details field
5. For an authorized order, provide the order email, complete required fields, review the service terms, and add the configured service to the cart. Preserve the configured total and order acknowledgment; an added cart item is not an accepted or executed run.

For custom workflows, use [EstiMate](https://cloud.ginkgo.bio/estimate). Its preliminary estimate is separate from ordering a catalog service. Do not report submission, payment, acceptance, execution, or QC success without the corresponding confirmation.

## Access and automation boundary

Use the public [catalog](https://cloud.ginkgo.bio/protocols) and the site's sign-in flow when account access is needed. For access or template discrepancies, use the official [contact page](https://www.ginkgo.bio/contact-us).

This skill covers the browser storefront. No public Cloud Lab submission API, SDK,
CLI, authentication-token contract, or pagination contract was identified in the
official material reviewed on 2026-09-30. The `/protocols`, `/estimate`, `/art`,
and `/gallery` URLs are web pages, not documented REST endpoints. Ginkgo's
[Catalyst software](https://www.ginkgo.bio/product/software) advertises REST
integration for installed automation systems; that is not a published Cloud Lab
ordering contract. Do not invent programmatic order calls.

## Interpreting results

- A280 concentration depends on the construct's extinction coefficient and eluate background; it does not establish purity or binding activity. Use the LabChip tier when size/purity data are required.
- HiBiT reports a tag-associated signal relative to a standard. Preserve background subtraction, calibration units, matrix controls, and QC flags when comparing constructs.
- Do not equate an expression, onboarding, or thermal-stability result with functional activity. Record the specific assay readout and its controls alongside any ranking.

From K-Dense-AI/scientific-agent-skills, MIT.

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